Journal: World Journal of Surgical Oncology

Article Title: The expression of stromal biomarkers in small papillary thyroid carcinomas

doi: 10.1186/s12957-022-02805-w

Figure Lengend Snippet: Immunohistochemical examples of various biomarkers and scores. A shows Col1 in PTC with slight expression and intensity in the tumor stroma. Normal location. B shows Col1 in PTC with strong expression and intensity in the tumor stroma. Normal location. C shows Col4 in a lymph node with no expression in the metastatic tumorous tissue. Normal expression and location in the normal lymphatic tissue nearby. D shows Col4 in PTC with strong distribution and intensity in the basement membrane and tumor stroma. Abnormal expression and location of the collagen type IV fibers. E shows a-SMA in PTC with slight distribution and intensity. Normal location. F shows a-SMA in PTC with medium distribution and intensity. Abnormal location. G shows MMP-9 in PTC with slight distribution and intensity. Normal location. H shows MMP-9 in PTC with strong distribution and intensity. Abnormal location. Pictures were taken using the Panoramic P250 FIII slide scanner (3DHISTECH) and reproduced using CaseViewer (3DHISTECH) at a 50-μm scale

Article Snippet: Five-micrometer thick, paraffin-embedded tissue slices were cut and stained using four different antibodies: anti-Col1 antibody (rabbit polyclonal ab34710, Abcam, Cambridge, UK), anti-Col4 antibody (mouse monoclonal antibody, BM4067, OriGene, Maryland, USA), anti-aSMA antibody (rabbit polyclonal ab5694, Abcam, Cambridge, UK), and anti-MMP9 antibody (rabbit polyclonal ab38898, Abcam, Cambridge, UK).

Techniques: Immunohistochemical staining, Expressing

Journal: World Journal of Surgical Oncology

Article Title: The expression of stromal biomarkers in small papillary thyroid carcinomas

doi: 10.1186/s12957-022-02805-w

Figure Lengend Snippet: Scoring system for Col1, Col4, a-SMA, and MMP-9—distribution score (percentage) in the tissue

Article Snippet: Five-micrometer thick, paraffin-embedded tissue slices were cut and stained using four different antibodies: anti-Col1 antibody (rabbit polyclonal ab34710, Abcam, Cambridge, UK), anti-Col4 antibody (mouse monoclonal antibody, BM4067, OriGene, Maryland, USA), anti-aSMA antibody (rabbit polyclonal ab5694, Abcam, Cambridge, UK), and anti-MMP9 antibody (rabbit polyclonal ab38898, Abcam, Cambridge, UK).

Techniques: Expressing, Staining

Journal: World Journal of Surgical Oncology

Article Title: The expression of stromal biomarkers in small papillary thyroid carcinomas

doi: 10.1186/s12957-022-02805-w

Figure Lengend Snippet: Comparison of the mean distribution scores ( Y -axis) in the normal and tumorous tissues divided by the tumor subgroups, non-metastatic (N0T) vs. metastatic (N1T) ( X -axis). Col1 ( a ) and Col4 ( b ) are significantly higher in the normal tissue. a-SMA ( c ) and MMP9 ( d ) are significantly higher in the cancer tissue. Black bars represent the normal tissue. Gray bars represent the tumorous tissue. ** p <0.01, *** p <0.001

Article Snippet: Five-micrometer thick, paraffin-embedded tissue slices were cut and stained using four different antibodies: anti-Col1 antibody (rabbit polyclonal ab34710, Abcam, Cambridge, UK), anti-Col4 antibody (mouse monoclonal antibody, BM4067, OriGene, Maryland, USA), anti-aSMA antibody (rabbit polyclonal ab5694, Abcam, Cambridge, UK), and anti-MMP9 antibody (rabbit polyclonal ab38898, Abcam, Cambridge, UK).

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: The expression of stromal biomarkers in small papillary thyroid carcinomas

doi: 10.1186/s12957-022-02805-w

Figure Lengend Snippet: Mean location, distribution, and total scores in Col1, Col4, a-SMA, and MMP-9

Article Snippet: Five-micrometer thick, paraffin-embedded tissue slices were cut and stained using four different antibodies: anti-Col1 antibody (rabbit polyclonal ab34710, Abcam, Cambridge, UK), anti-Col4 antibody (mouse monoclonal antibody, BM4067, OriGene, Maryland, USA), anti-aSMA antibody (rabbit polyclonal ab5694, Abcam, Cambridge, UK), and anti-MMP9 antibody (rabbit polyclonal ab38898, Abcam, Cambridge, UK).

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: The expression of stromal biomarkers in small papillary thyroid carcinomas

doi: 10.1186/s12957-022-02805-w

Figure Lengend Snippet: Comparison of the mean distribution (dark gray bars) and total scores (light gray bars) ( Y -axis) divided by the different tumor groups (N0T vs. N1T) and the metastatic lymph nodes (N1N) ( X -axis). Col1 ( a ) is significantly higher in the non-metastatic tumors (N0T) compared with the metastatic ones (N1T). Col4 ( b ) is significantly higher in the non-metastatic tumors (N0T) compared with the metastatic ones (N1T) and even with the metastatic lymph nodes (N1N). * p <0.05, *** p <0.001

Article Snippet: Five-micrometer thick, paraffin-embedded tissue slices were cut and stained using four different antibodies: anti-Col1 antibody (rabbit polyclonal ab34710, Abcam, Cambridge, UK), anti-Col4 antibody (mouse monoclonal antibody, BM4067, OriGene, Maryland, USA), anti-aSMA antibody (rabbit polyclonal ab5694, Abcam, Cambridge, UK), and anti-MMP9 antibody (rabbit polyclonal ab38898, Abcam, Cambridge, UK).

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: The expression of stromal biomarkers in small papillary thyroid carcinomas

doi: 10.1186/s12957-022-02805-w

Figure Lengend Snippet: Scoring system for Col1, Col4, a-SMA, and MMP-9—location score

Article Snippet: Five-micrometer thick, paraffin-embedded tissue slices were cut and stained using four different antibodies: anti-Col1 antibody (rabbit polyclonal ab34710, Abcam, Cambridge, UK), anti-Col4 antibody (mouse monoclonal antibody, BM4067, OriGene, Maryland, USA), anti-aSMA antibody (rabbit polyclonal ab5694, Abcam, Cambridge, UK), and anti-MMP9 antibody (rabbit polyclonal ab38898, Abcam, Cambridge, UK).

Techniques: Expressing

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.

doi: 10.1016/j.biopha.2020.110321

Figure Lengend Snippet: Fig. 4. Effects of Ibrolipim on the mRNA and protein expression of redox en- zymes. (A) Representative protein band images detected by Western blot. (B and F) The protein and mRNA expression of Nox-4. (C and G) The protein and mRNA expression of SOD1. (D and H) The protein and mRNA expression of CAT. (E and I) The protein and mRNA expression of GPX1. The relative protein and mRNA levels are normalized to internal control β-actin. Data are mean ± SD (n = 5 per group). ** P < 0.01 vs. CD group; # P < 0.05, ## P < 0.01 vs. HSFD group. CD, normal control diet; HSFD, high sucrose and fat diet; Nox-4, nicotinamide-adenine dinucleotide phosphate oxidase 4; SOD1, superoxide dismutase 1; CAT, catalase; GPX1, glutathione peroxidase 1.

Article Snippet: The sections were incubated with primary antibodies against Nox-4, SOD1, CAT, GPX1, collagen type IV alpha 1 (COL4A1), TGF-β1 or matrix metalloproteinase 2 (MMP-2) (1:100 rabbit polyclonal antibodies from Boster Biological Technology Co., China).

Techniques: Expressing, Western Blot, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.

doi: 10.1016/j.biopha.2020.110321

Figure Lengend Snippet: Fig. 5. Effects of Ibrolipim on the protein expression of redox enzymes detected by immunohistochemistry. (A) Representative immunohistochemical staining for Nox-4, SOD1, CAT and GPX1 (Original magnification ×400, Bar =20 μm). The expression of Nox-4 (B), SOD1 (C), CAT (D) and GPX1 (E) is quantitatively calculated as the percentage of positive area to total area (%). Data are mean ± SD (n = 5 per group). ** P < 0.01 vs. CD group; # P < 0.05, ## P < 0.01 vs. HSFD group. CD, normal control diet; HSFD, high sucrose and fat diet; Nox-4, nicotinamide-adenine dinucleotide phosphate oxidase 4; SOD1, superoxide dismutase 1; CAT, catalase; GPX1, glutathione peroxidase 1.

Article Snippet: The sections were incubated with primary antibodies against Nox-4, SOD1, CAT, GPX1, collagen type IV alpha 1 (COL4A1), TGF-β1 or matrix metalloproteinase 2 (MMP-2) (1:100 rabbit polyclonal antibodies from Boster Biological Technology Co., China).

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis.

doi: 10.1016/j.biopha.2020.110321

Figure Lengend Snippet: Fig. 8. Proposed mechanisms of HSFD-induced renal injury and the protective effects of Ibrolipim. The black arrows represent the de- teriorative pathogenesis of nutrient stress, and the red arrows and cross indicate the re- noprotective effects of Ibrolipim. Two-way ar- rows mean correlation, and the upward arrows imply activation while the downward arrows imply inhibition. ROS, reactive oxygen species; MDA, malondialdehyde; GSH, reduced glu- tathione; T-AOC, total antioxidant capacity; ECM, extracellular matrix; Nox-4, nicotina- mide-adenine dinucleotide phosphate oxidase 4; SOD1, superoxide dismutase 1; CAT, cata- lase; GPX1, glutathione peroxidase 1; TGF-β1, transforming growth factor-β1; MMP-2, matrix metalloproteinase 2 (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

Article Snippet: The sections were incubated with primary antibodies against Nox-4, SOD1, CAT, GPX1, collagen type IV alpha 1 (COL4A1), TGF-β1 or matrix metalloproteinase 2 (MMP-2) (1:100 rabbit polyclonal antibodies from Boster Biological Technology Co., China).

Techniques: Activation Assay, Inhibition

Journal: Brain Communications

Article Title: ‘Raisin bread sign’ feature of pontine autosomal dominant microangiopathy and leukoencephalopathy

doi: 10.1093/braincomms/fcad281

Figure Lengend Snippet: The pedigree charts of three PADMAL families (Family 1–3) and the identified variant in COL4A1 3′-untranslated region (UTR). ( A – C ) Square symbols indicate men and round symbols indicate women. Closed symbols indicate affected individuals proven by clinical histories or MRI. Diagonal lines indicate deceased individuals. Open symbols indicate individuals without PADMAL proven clinically or radiologically. Arrows indicate probands. The pedigree charts of F1 ( A ) and F2 ( B ) show autosomal dominant inheritance pattern while F3 ( C ) does not. ( D ) Sanger sequencing was performed with the proband (F1-IV-2) in F1. The heterozygous variant of COL4A1 located in c.*33T > A was identified, which is indicated by an arrowhead. ( E ) The healthy control in F1 (F1-IV-1) showed no variant in COL4A1 3′UTR in Sanger sequencing.

Article Snippet: Immunohistochemistry was performed with rabbit polyclonal antibody against COL4A1 (1:100, SAB4300825, Sigma-Aldrich, St Louis, MO) and α-smooth muscle actin (1:250, ab7817, Abcam, Cambridge, MA) through the use of a Ventana BenchMark GX automated slide staining system (Ventana Medical Systems, Tucson, AZ) in accordance with the instructions of the manufacturer.

Techniques: Variant Assay, Sequencing

Journal: Brain Communications

Article Title: ‘Raisin bread sign’ feature of pontine autosomal dominant microangiopathy and leukoencephalopathy

doi: 10.1093/braincomms/fcad281

Figure Lengend Snippet: Histopathology in F1-IV-3. ( A , B ) Gross finding of the brain of F1-IV-2. Atrophies of frontotemporal lobes and brainstem, and the atherosclerosis of the basilar artery were observed. ( C ) The focused image of the pons on fluid attenuated inversion recovery. ( D, E ) Haematoxylin and eosin staining ( D ) and Klüver–Barrera staining ( E ) sections of the pons showed multiple infarctions and existing lesions revealed by MRI. ( F ) Hyalinized vessels in the non-ischaemic lesions had thickened media tunica. ( G ) The pontine ischaemic lesions did not include vessels with thick media tunica. ( H–J ) Vessels in ischaemic lesions ( H ) showed immunopositivity of COL4A1 ( I ) and α-smooth muscle actin ( J ) around the vessels. ( K–M ) Vessels in the non-ischaemic areas ( K ) was hyalinized with thick media tunica, which were immunopositive for COL4A1 ( L ) and α-smooth muscle actin ( M ). Scale bars: ( D, E ) 1 mm, ( F, G ) 200 μm, ( H–J, L, M ) 20 μm, ( K ) 100 μm.

Article Snippet: Immunohistochemistry was performed with rabbit polyclonal antibody against COL4A1 (1:100, SAB4300825, Sigma-Aldrich, St Louis, MO) and α-smooth muscle actin (1:250, ab7817, Abcam, Cambridge, MA) through the use of a Ventana BenchMark GX automated slide staining system (Ventana Medical Systems, Tucson, AZ) in accordance with the instructions of the manufacturer.

Techniques: Histopathology, Staining